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Full View Required;. The fidelity of the Cas nuclease, i. RNA (sgRNA)–matching genomic target locus as compared to OFF-target sites exhibiting partial complementarity to the RNA guide, is a key parameter to be considered for CRISPR applications. Monosomes translate proteins in neurons.
Like all other cells, neurons use different proteins to process information and respond to stimuli. To meet the huge demands for new proteins in their large and complex cell volume, neurons have moved the protein templates—messenger RNAs (mRNAs)—and the protein synthesis machines—ribosomes—out to synapses to make proteins locally.
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